US10337021B2 – July 2, 2019 – Methods of modulating seed and organ size in plants

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Inventors :

Michael Bevan; Jack Dumenil

Owner :

A01H5 / 08; A01H1 / 00; A01H5 / 00; C07K14 / 415; C12N15 / 82

Application Number :

US15035657

Document Number :

US10337021B2

Priority Date :

November 11, 2013

Filing Date :

November 5, 2014

Date of Grant/ Publication :

July 2, 2019

Class :

PLANT BIOSCIENCE LIMITED

Abstract

This invention relates to the expression a DA1 protein with a mutation that disrupts or inactivates the LIM domain or the LIM-like domain within cells of a plant. This may increase the yield or enhance a yield-related trait of the plant. Methods, plants and plant cells are provided.

Claim(s)

1. A method of increasing the yield of a plant comprising: expressing a nucleic acid encoding a DA1 protein within cells of said plant, wherein the DA1 protein comprises an amino acid sequence having at least 90% sequence identity to one or more of SEQ NOS: 4 to 27, wherein the amino acid sequence of the DA1 protein comprises a mutation that disrupts or inactivates the LIM domain of the DA1 protein, wherein the LIM domain of the DA1 protein comprises one or more sequence alterations relative to the wild-type LIM domain of SEQ ID NO: 1 which inactivate the LIM domain, wherein the wild-type LIM domain comprises two Zn finger motifs and the sequence alterations abolish one or both Zn finger motifs, wherein the plant has increased yield compared to a plant without the mutation grown in the same conditions, and wherein increased yield comprises increased organ size and/or seed size.;
3. A method of producing a plant with an increased yield comprising: introducing into a plant cell a heterologous nucleic acid which encodes a DA1 protein, wherein the amino acid sequence of the DA1 protein comprises a mutation that disrupts or inactivates the LIM domain of the DA1 protein, or introducing a mutation into the nucleotide sequence of a plant cell which encodes a DA1 protein, such that the LIM domain of the DA1 protein is disrupted or inactivated, and regenerating the plant from the plant cell, wherein the plant has increased yield compared to a plant without the mutations grown in the same conditions, wherein increased yield comprises increased organ size and/or seed size; wherein the DA1 protein comprises an amino acid sequence having at least 90% sequence identity to one or more of SEQ NOS: 4 to 27, wherein the disrupted or inactivated LIM domain of the DA1 protein comprises one or more sequence alterations relative to the wild-type LIM domain of SEQ ID NO: 1 which inactivate the LIM domain, and wherein the wild-type LIM domain comprises two Zn finger motifs and the sequence alterations abolish one or both Zn finger motifs.;
16. A plant cell comprising a heterologous nucleic acid encoding a DA1 protein having a disrupted or inactivated LIM domain, wherein the DA1 protein comprises an amino acid sequence having at least 90% sequence identity to any one of SEQ NOS: 4 to 27, wherein the LIM domain of the DA1 protein comprises one or more sequence alterations relative to the wild-type LIM domain of SEQ ID NO: 1 which inactivate the LIM domain, and wherein the wild-type LIM domain comprises two Zn finger motifs and the sequence alterations abolish one or both Zn finger motifs.

Summary

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