US10337024B2 – July 2, 2019 – Plants with enhanced photosynthesis and methods of manufacture thereof

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Inventors :

Danny J. Schnell; Mine O. Canakci; Bibin Paulose; Michelle DaCosta Inguagiato

Owner :

THE UNIVERSITY OF MASSACHUSETTS, MASSACHUSETTS

Application Number :

US15109349

Document Number :

US10337024B2

Priority Date :

December 24, 2014

Filing Date :

December 24, 2014

Date of Grant/ Publication :

July 2, 2019

Class :

C12N15 / 82; C07K14 / 405

Abstract

A transgenic plant having enhanced photosynthesis is disclosed. The transgenic plant is transformed with a transgenic polynucleotide encoding a heterologous bicarbonate transporter. The bicarbonate transporter can be from an algae or a cyanobacterial species. The transgenic polynucleotide comprises a nucleic acid sequence encoding the bicarbonate transporter under the control of a functional plant promoter and optionally includes a chloroplast envelope targeting peptide heterologous to the bicarbonate transporter. Methods of making the transgenic plant and transgenic polynucleotide are disclosed.

Claim(s)

1. A transgenic plant transformed with a recombinant DNA construct comprising a plant-expressible transcription regulatory sequence operatively linked to a polynucleotide encoding an algal CCP1 or CCP2 polypeptide comprising an amino acid sequence that is at least 80% homologous to SEQ ID NO: 6, wherein the transgenic plant has a CO 2 assimilation rate at least 5% higher than a plant of the same species not transformed with the recombinant DNA construct and a reduced transpiration rate at least 5% lower than a plant of the same species not transformed with the recombinant DNA construct.;
6. A method of producing a transformed plant having enhanced photosynthesis, the method comprising transforming a plant cell with a recombinant polynucleotide comprising a nucleic acid sequence encoding an algal CCP1 or CCP2 polypeptide, comprising an amino acid sequence that is at least 80% homologous to SEQ ID NO:6, operatively linked to a plant-expressible promoter, wherein the nucleic acid sequence encoding the algal CCP1 or CCP2 polypeptide is further operatively linked to a transcription terminator; growing a plant from the plant cell until the plant produces seed; and selecting seeds from a plant in which the transgenic plant has a CO 2 assimilation rate higher than a plant of the same species not comprising the algal CCP1 or CCP2 polypeptide and a reduced transpiration rate lower than a plant of the same species not comprising the algal CCP1 or CCP2 polypeptide.

Summary

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