US10364416B2 – July 30, 2019 – Cannabichromenic acid synthase from cannabis sativa

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Inventors :

Jonathan E. Page; Jason M. Stout

Owner :

NATIONAL RESEARCH COUNCIL OF CANADA, CANADA

Application Number :

US15321585

Document Number :

US10364416B2

Priority Date :

June 29, 2015

Filing Date :

June 29, 2015

Date of Grant/ Publication :

July 30, 2019

Class :

C12N9 / 88; C12N9 / 02; C12P17 / 06; C12N15 / 82; C07K16 / 40; C12N15 / 52

Abstract

Nucleic acid molecules from cannabis have been isolated and characterized and encode polypeptides having cannabichromenic acid synthase activity. Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.

Claim(s)

1. A method of modulating levels of cannabinoid compounds in an organism, cell or tissue, said method comprising: a) introducing a nucleic acid molecule comprising: i) a nucleotide sequence complementary to a polynucleotide encoding at least a portion of a polypeptide having the sequence set for in SEQ ID NO: 2 or 6 or a polypeptide having at least 99.5% sequence identity to SEQ ID NO: 2 or 6; or ii) a nucleotide sequence complementary to a polynucleotide having at least, greater than or about 99.5% sequence identity to at least a portion of SEQ ID NO: 1 or 5; into the organism, cell or tissue to decrease the level of a cannabinoid compound, wherein the portion comprises greater than, at least or about 15 contiguous nucleotides, and wherein the nucleic acid molecule is comprised in a construct, to silence cannabichromenic acid synthase gene in the organism, cell or tissue to decrease the level of a cannabinoid compound; or b) introducing a nucleic acid molecule or construct comprising said nucleic acid molecule, and expressing or over-expressing a polypeptide having cannabichromenic acid synthase activity encoded by said nucleic acid molecule, said nucleic acid molecule comprising i) a nucleotide sequence encoding a polypeptide having the sequence set for in SEQ ID NO: 2 or 6 or a polypeptide having at least 99.5% sequence identity to SEQ ID NO: 2 or 6; ii) a nucleotide sequence having at least, greater than or about 99.5% sequence identity to SEQ ID NO: 1 or 5; or iii) a nucleotide sequence having at least, greater than or about 90% sequence identity to SEQ ID NO: 8 or 9; in the organism, cell or tissue to increase the level of a cannabinoid compound.

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