US10400222B2 – September 3, 2019 – Desaturase nucleic acids and polypeptides

Please complete the required fields.




Inventors :

Monika Bielecka; Filip Kaminski; Thilo Winzer; Ian Graham

Owner :

THE UNIVERSITY OF YORK, UNITED KINGDOM

Application Number :

US15101357

Document Number :

US10400222B2

Priority Date :

January 1, 1970

Filing Date :

December 8, 2014

Date of Grant/ Publication :

September 3, 2019

Class :

C12N15 / 82; C12N9 / 02; A01H5 / 10; A01H5 / 12; A23D9 / 00; C11B1 / 06

Abstract

The disclosure relates to delta ( 12 ) and delta ( 15 ) desaturases and their use in the modification of oil content in hemp.

Claim(s)

1. A modified Cannabis spp plant generated by mutagenesis with a chemical mutagen or physical mutagen, comprising: a modified delta-12 desaturase gene comprising a termination codon at position 167 of SEQ ID NO: 3, resulting in a truncated and non-functional delta-12 desaturase polypeptide in the modified Cannabis spp plant, wherein the modified delta-12 desaturase gene comprising a termination codon at position 167 of SEQ ID NO: 3 is homozygous, and wherein the modified plant has an oleic acid content of between 70-85% of the total oil content of the modified plant.;
7. A modified Cannabis spp plant generated by mutagenesis with a chemical mutagen or physical mutagen, comprising: a modified delta-12 desaturase gene comprising amino add substitutions at serine 218 and serine 375 of SEQ ID NO: 3 with proline, resulting in the modified Cannabis spp plant wherein the modified delta-12 desaturase gene comprising amino acid substitutions at serine 218 and serine 375 of SEQ ID between 70-85% of the total oil content of the modified plant.

Summary

No Comments

Leave a comment

Sorry, you must be logged in to post a comment. Login