US10450578B2 – October 22, 2019 – Modulation of expression of acyltransferases to modify hydroxycinnamic acid content

Please complete the required fields.




Inventors :

Laura E. Bartley; Pamela Ronald; Henrik Vibe Scheller

Owner :

UNITED STATES DEPARTMENT OF ENERGY, DISTRICT OF CO THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, CALIF

Application Number :

US15652115

Document Number :

US10450578B2

Priority Date :

July 17, 2017

Filing Date :

July 17, 2017

Date of Grant/ Publication :

October 22, 2019

Class :

C12N15 / 82; C12N9 / 10

Abstract

The invention provides methods of engineering plants to modulate hydroxycinnamic acid content. The invention additionally provides compositions and methods comprising such plants.

Claim(s)

claim 1: 1. A method of engineering a plant to decrease the ferulic acid content in the plant, the method comprising: introducing an expression cassette into the plant, wherein the expression cassette comprises a polynucleotide encoding an AT15 acyltransferase, and wherein the acyltransferase has at least 95% amino acid sequence identity to SEQ ID NO: 4, culturing the plant under conditions in which the acyltransferase is expressed; and selecting a plant that has a decreased ferulic acid content compared to a wildtype plant.;
claim 2: 8. A plant cell comprising a polynucleotide encoding an AT15 acytransferase having at least 95% amino acid sequence identity to SEQ ID NO: 4, operably linked to a heterologous promoter; or a plant comprising a heterologous polynucleotide encoding an AT15 acyltransferase having at least 95% amino acid sequence identity to SEQ ID NO: 4.

Summary

No Comments

Leave a comment

Sorry, you must be logged in to post a comment. Login