US20190099697A1 – April 4, 2019 – Methods for Removing Pesticides from Cannabinoid Extract Oils Using Countercurrent Partition Chromatography

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Inventors :

Erwin Sibal

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Priority Date :

October 3, 2018

Filing Date :

October 3, 2018

Date of Grant/ Publication :

April 4, 2019

Class :

; B01D15 / 30; B01D1 / 22; B01D11 / 04


The disclosure provides methods and reagents for removing pesticides or pesticide residues from plant matter such as cannabis plant matter. The method uses Countercurrent Partition Chromatography (CPC).


1 . A method for removing pesticides from an oil extract of plant matter, wherein the plant matter contains one or more different kinds of pesticides, the method comprising: (1) The step of washing, soaking, or washing and soaking the plant matter with ethanol, thereby extracting cannabinoids from the plant matter, and resulting in an oil extract that is dissolved or dispersed in the ethanol, (2) The step of removing the ethanol from the oil extract, wherein the removing is optionally with a rotary evaporator, resulting in an ethanol-free crude oil extract, (3) Dissolving the crude ethanol-free oil extract in hexane, resulting in a hexane solution that is optionally at a ratio of 1:5 ethanol-free oil extract/hexane (vol./vol.), (4) The step of preparing the stationary phase that comprises 3 parts ethanol and 1 part deionized water, (5) The step of adjusting the pH of the stationary phase to effect acid-induced ionization of an ionizable group on at least one of the one or more different pesticides to produce a pH-adjusted liquid composition, (6) The step running the stationary phase into the system to equilibrium running the pH-adjusted liquid composition in a Countercurrent Partition Chromatography (CPC) cartridge, wherein once the stationary phase is in place, the mobile phase is run into the system to equilibrium wherein the cartridge is rotated, and wherein the CPC is run in an ascending mode that, produces an upper phase of a binary solvent mixture, and (7) The step of collecting the upper phase of the binary solvent mixture.


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