US20190134532A1 – May 9, 2019 – Methods of Purifying Cannabinoids, Compositions and Kits Thereof

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Inventors :

Xavier Nadal Roura

Owner :

PHYTOPLANT RESEARCH S.L.

Application Number :

US16239002

Document Number :

US20190134532A1

Priority Date :

January 3, 2019

Filing Date :

January 3, 2019

Date of Grant/ Publication :

May 9, 2019

Class :

; B01D11 / 02; C07D311 / 80; B01D15 / 18; C07C37 / 72; G01N30 / 88; C07C51 / 48; B01D11 / 04; C07C37 / 00

Abstract

The present specification discloses methods of purifying one or more cannabinoids from a plant material, purified cannabinoids and pharmaceutical compositions comprising one or more cannabinoids produced by the disclosed method, methods and uses for treating a disease or condition employing such purified cannabinoids and pharmaceutical compositions.

Claim(s)

1 . A method of purifying one or more cannabinoids from a plant material, the method consisting essentially of the following steps: a) incubating the plant material with a non-polar solvent selected from the group consisting of petroleum ether, pentane, hexane and heptane to form a first solvent mixture which extracts the one or more cannabinoids from a plant material; b) reducing the volume of the first solvent mixture to about 50% or less of the original volume of the first solvent mixture in step (a) thereby concentrating the one or more cannabinoids; c) incubating the reduced first solvent mixture at a temperature range of between about ?70° C. to about 40° C. to crystalize the one or more cannabinoids; d) incubating the one or more crystalized cannabinoids with the non-polar solvent, wherein the non-polar solvent is the same non-polar solvent as in step (a) to form a second solvent mixture, thereby dissolving at least 50% of the one or more crystalized cannabinoids; and e) incubating the second solvent mixture at a temperature range of between about ?70° C. to about 40° C. to crystalize the one or more cannabinoids, and f) washing the crystallized one or more cannabinoids by dissolving in ethanol, methanol, isopropylic alcohol or acetone, filtering, evaporating to dryness and recrystallizing using the same non-polar solvent as in step a), thereby resulting in the purification of one or more cannabinoids.;
9 . A method of purifying a cannabinoid from a plant material, the method consisting essentially of the following steps: a) incubating the plant material with a solvent selected from the group consisting of pentane, hexane, heptane, cyclohexane, petroleum ethers, dicloromethane, tricloromethane, tethrahydrofurane, diethyl ether, ethanol, methanol, isopropanol, acetone, acetonitrile, ethyl acetate, butane, propane, refrigerant gas 1,1,1,2-Tetrafluoroethane (R134a), liquid CO 2 , subcritical CO 2 or supercritical CO 2 or mixes of these solvents to form a first solvent mixture which extracts the one or more cannabinoids from a plant material; b) filtering, decanting or centrifuging the first solvent mixture; c) reducing the volume of the first solvent mixture to dryness thereby concentrating the one or more cannabinoids in a first extract; d) incubating the first extract with a non-polar solvent selected from the group consisting of petroleum ether, pentane, hexane and heptane to form a second solvent mixture which extracts the one or more cannabinoids from the first extract; e) filtering, decanting or centrifuging the second solvent mixture; f) incubating the second solvent mixture at a temperature range of between about ?70° C. to about 40° C. to crystalize the one or more cannabinoids; g) collecting the one or more crystalized cannabinoids in step (f) using filtration that results in a collection of a crystalline product and a mother liquor; h) incubating the one or more crystalized cannabinoids of step (g) with the non-polar solvent wherein the non-polar solvent is the same non-polar solvent as in step (d) to form a third solvent mixture, wherein the third solvent mixture dissolves at least 50% of the one or more crystalized cannabinoids; i) incubating the third solvent mixture at a temperature range of between about ?70° C. to about 40° C. to crystalize the one or more cannabinoids, and then washing the crystallized one or more cannabinoids by dissolving in ethanol, methanol, isopropylic alcohol or acetone, filtering, evaporating to dryness and recrystallizing using the same non-polar solvent as in step d); and j) collecting the one or more crystalized cannabinoids of step (i) using filtration that results in a collection of a crystalline product and a mother liquor, thereby resulting in the purification of one or more cannabinoids

Summary

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